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Jingdong book

实用现代病理学技术

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实用现代病理学技术

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现代科学技术的发展促进了医学的发展,病理学也是如此。从20世纪50年代的免疫荧光技术、免疫酶技术,到现在的分子生物学技术、基因技术和蛋白质技术,以及计算机技术在病理学科的广泛应用,病理学的发展和建设融人诸多新的观念和理论。进入21世纪以来,病理新技术、新产品、新设备的不断推陈出新,推动着病理学科的进步,使之逐步朝着自动化、信息化、智能化、标准化的方向发展。
北京市病理技术学组组织,会同中国医学科学院、北京大学、首都医科大学、卫生部和解放军系统所属各医院的病理技术工作者编写了这本《实用现代病理学技术》。《实用现代病理学技术》编者都是从事病理技术的专家,有的从事病理技术工作40余年,有着坚实的病理基础专业知识和丰富的实践工作经验。《实用现代病理学技术》共33章240余节,从组织接收、取材、固定、脱水、包埋、常规切片染色、特殊染色、免疫组织化学到分子生物学技术,从手工操作到全流程自动化设备,从经验体会到质量管理进行了系统介绍,并附有大量的图片、涵盖了现代病理技术的发展、现代病理技术的应用、现代病理技术新型设备的介绍等全部内容,是一本适合于各级医院病理科、医学院校、科研院所等从事病理诊断的初级医师、实验室研究人员、病理技师及管理人员的工具书及教科书。
《实用现代病理学技术》的编写得到了著名病理学家刘彤华院士的热诚支持和指导,并予作序,体现了老一代病理学家对病理技术学科建设和发展的重视与支持。
Catalogue

第一章 病理实验室工作流程及日常工作质量控制
第一节 病理实验室工作流程
第二节 病理实验室日常工作质量控制

第二章 组织固定与固定液
第一节 组织固定
第二节 组织固定液

第三章 组织标本取材
第一节 组织标本常规取材的概念及要求
第二节 用于分子生物学研究及电镜检查的组织取材
第三节 常见的外科大体标本组织常规取材简介

第四章 组织脱水、透明、浸蜡
第一节 组织脱水
第二节 组织透明
第三节 组织浸蜡
第四节 全密闭程序控制自动组织脱水机
第五节 半封闭式自动组织脱水机
第六节 手工脱水
第七节 组织快速脱水法
第八节 全自动快速微波组织处理仪
第九节 全自动超高速固定脱水浸透装置(Histra-QS)

第五章 组织包埋制作技术
第一节 包埋的定义
第二节 组织包埋的发展及现状
第三节 全自动组织包埋、冷台一体机
第四节 包埋模具的选择
第五节 包埋石蜡的选择
第六节 组织包埋的方法
第七节 组织包埋应注意的问题

第六章 石蜡组织切片制作技术
第一节 石蜡组织切片制作概述
第二节 石蜡组织切片机的种类
第三节 石蜡切片辅助设备
第四节 传统切片刀
第五节 一次性切片刀片类型与选择
第六节 轮转式石蜡切片机
第七节 平推式石蜡切片机
第八节 硬(骨)组织石蜡切片机
第九节 切片中易出现的问题及解决办法

第七章 苏木精一伊红染色技术
第一节 苏木精染色原理及配制方法
第二节 伊红染色原理及配制方法
第三节 苏木精伊红染色的分化控制与返蓝
第四节 苏木精伊红染色手工操作程序及注意事项
第五节 程序控制的全自动组织切片染色机
第六节 手工封片
第七节 全自动组织切片封片机
第八节 程序控制的全自动组织切片染色封片一体机
第九节 玻片标签的打印、排序及核对
第十节 HE切片的规范技术标准
第十一节 HE染色易出现的问题、原因及补救方法

第八章 冷冻切片制作技术
第一节 冷冻切片的意义
第二节 冷冻切片的发展史
第三节 恒温冷冻切片机的结构与功能
第四节 冷冻切片组织的取材
第五节 恒温冷冻切片机的温度设置
第六节 冷冻切片的制作方法
第七节 冷冻切片的固定和染色
第八节 恒温冷冻切片机的使用注意事项及日常维护

第九章 常用特殊染色技术
第一节 结缔组织多色染色
第二节 胶原纤维染色
第三节 网状纤维染色
第四节 弹力纤维染色
第五节 横纹肌染色
……
第十章 免疫组织化学染色技术
第十一章 肾活栓制作技术
第十二章 肌肉组织活检及染色技术
第十三章 骨组织标本制作技术
第十四章 肝活检组织制作技术
第十五章 皮肤组织活检制作技术
第十六章 特殊组织制作技术
第十七章 病理尸检常规技术
第十八章 电子显微镜技术
第十九章 核酸原位杂交技术
第二十章 荧光原位杂交技术
第二十一章 EBER检测(原位杂交)技术
第二十二章 基因重排检测技术
第二十三章 PCR技术
第二十四章 突变检测技术
第二十五章 细胞病理学技术
第二十六章 生物芯片及组织芯片制作技术
第二十七章 动物组织标本制作技术
第二十八章 病理实验室的设置、设备及常用试剂介绍
第二十九章 新仪器、新设备介绍
第三十章 环保试剂的应用
第三十一章 档案资料管理
第三十二章 常用仪器设备维护与保养
第三十三章 染色技术术语及病理名词解释
参考文献

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